Structural highlights
Publication Abstract from PubMed
Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.
Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A.,Omura K, Aiba Y, Onoda H, Stanfield JK, Ariyasu S, Sugimoto H, Shiro Y, Shoji O, Watanabe Y Chem Commun (Camb). 2018 Jul 12;54(57):7892-7895. doi: 10.1039/c8cc02760a. PMID:29845154[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Omura K, Aiba Y, Onoda H, Stanfield JK, Ariyasu S, Sugimoto H, Shiro Y, Shoji O, Watanabe Y. Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A. Chem Commun (Camb). 2018 Jul 12;54(57):7892-7895. doi: 10.1039/c8cc02760a. PMID:29845154 doi:http://dx.doi.org/10.1039/c8cc02760a
