Structural highlights
Publication Abstract from PubMed
Anthraquinones, a widely distributed class of aromatic natural products, are produced by a type II polyketide synthase system in the Gram-negative bacterium Photorhabdus luminescens. Heterologous expression of the antABCDEFGHI anthraquinone biosynthetic gene cluster in Escherichia coli identified AntI as an unusual lyase, catalysing terminal polyketide shortening prior to formation of the third aromatic ring. Functional in vitro and in vivo analysis of AntI using X-ray crystallography, structure-based mutagenesis, and molecular simulations revealed that AntI converts a defined octaketide to the tricyclic anthraquinone ring via retro-Claisen and Dieckmann reactions. Thus, AntI catalyses a so far unobserved multistep reaction in this PKS system.
Molecular mechanism of polyketide shortening in anthraquinone biosynthesis of Photorhabdus luminescens.,Zhou Q, Brauer A, Adihou H, Schmalhofer M, Saura P, Grammbitter GLC, Kaila VRI, Groll M, Bode HB Chem Sci. 2019 May 14;10(25):6341-6349. doi: 10.1039/c9sc00749k. eCollection 2019, Jul 7. PMID:31341589[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhou Q, Brauer A, Adihou H, Schmalhofer M, Saura P, Grammbitter GLC, Kaila VRI, Groll M, Bode HB. Molecular mechanism of polyketide shortening in anthraquinone biosynthesis of Photorhabdus luminescens. Chem Sci. 2019 May 14;10(25):6341-6349. doi: 10.1039/c9sc00749k. eCollection 2019, Jul 7. PMID:31341589 doi:http://dx.doi.org/10.1039/c9sc00749k
