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1kpa

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Revision as of 09:11, 30 October 2007 by OCA (Talk | contribs)
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1kpa, resolution 2.0Å

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PKCI-1-ZINC

Overview

The three-dimensional structure of protein kinase C interacting protein 1, (PKCI-1) has been solved to high resolution by x-ray crystallography using, single isomorphous replacement with anomalous scattering. The gene, encoding human PKCI-1 was cloned from a cDNA library by using a partial, sequence obtained from interactions identified in the yeast two-hybrid, system between PKCI-1 and the regulatory domain of protein kinase C-beta., The PKCI-1 protein was expressed in Pichia pastoris as a dimer of two, 13.7-kDa polypeptides. PKCI-1 is a member of the HIT family of proteins, shown by sequence identity to be conserved in a broad range of organisms, including mycoplasma, plants, and humans. Despite the ubiquity of this, protein sequence in nature, no distinct function has been shown for ... [(full description)]

About this Structure

1KPA is a [Single protein] structure of sequence from [Homo sapiens]. Structure known Active Sites: ZNA and ZNB. Full crystallographic information is available from [OCA].

Reference

Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins., Lima CD, Klein MG, Weinstein IB, Hendrickson WA, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5357-62. PMID:8643579

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