6qh1

Structural highlights

Function

[PCNA_SCHPO] This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. [SPD1_SCHPO] Regulates the ribonucleotide reductase activity through its mediation of the nuclear localization of suc22, the small subunit of the ribonucleotide reductase. Delays the progression of the G1-S phase transition, thereby ensuring the G1 phase is complete. Interacts with both p34 and the p34-p56 complex, although no direct inhibitory effect on the bound proteins has been demonstrated. The action of p14 may happen coincidentally with the cdc10 function or may happen downstream of this.^{[1] [2] [3] [4]}

References

1. ↑ Borgne A, Nurse P. The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast. J Cell Sci. 2000 Dec;113 Pt 23:4341-50. PMID:11069778
2. ↑ Liu C, Powell KA, Mundt K, Wu L, Carr AM, Caspari T. Cop9/signalosome subunits and Pcu4 regulate ribonucleotide reductase by both checkpoint-dependent and -independent mechanisms. Genes Dev. 2003 May 1;17(9):1130-40. Epub 2003 Apr 14. PMID:12695334 doi:http://dx.doi.org/10.1101/gad.1090803
3. ↑ Hakansson P, Dahl L, Chilkova O, Domkin V, Thelander L. The Schizosaccharomyces pombe replication inhibitor Spd1 regulates ribonucleotide reductase activity and dNTPs by binding to the large Cdc22 subunit. J Biol Chem. 2006 Jan 20;281(3):1778-83. doi: 10.1074/jbc.M511716200. Epub 2005, Nov 28. PMID:16317005 doi:http://dx.doi.org/10.1074/jbc.M511716200
4. ↑ Woollard A, Basi G, Nurse P. A novel S phase inhibitor in fission yeast. EMBO J. 1996 Sep 2;15(17):4603-12. PMID:8887552