| Structural highlights
5zch is a 4 chain structure with sequence from Japanese rice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , |
| Gene: | PP2C50, ABIL3, Os05g0537400, LOC_Os05g46040, OJ1741_B01.18, OSJNBa0052K01.2 (Japanese rice), PYL3, PYL10, RCAR3, Os02g0255500, LOC_Os02g15640, OSJNBa0052K15.19, P0613F08.1 (Japanese rice) |
| Activity: | Phosphoprotein phosphatase, with EC number 3.1.3.16 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[PYL3_ORYSJ] Involved in abscisic acid (ABA) signaling during seed germination and abiotic stress response. Acts as positive regulator of ABA-mediated inhibition of seed germination, and tolerance to drought and cold stresses (PubMed:26362328). Together with PP2C50 and SAPK10, may form an ABA signaling module involved in stress response (PubMed:28827170). Inhibits the protein phosphatases PP2C06 and PP2C09 when activated by abscisic acid (ABA) (PubMed:24743650).[1] [2] [3]
Publication Abstract from PubMed
Regulation of abscisic acid (ABA) signaling is crucial in balancing responses to abiotic stresses and retaining growth in planta. An ABA receptor (PYL/RCAR) and a protein phosphatase (PP2C), a co-receptor, form a complex upon binding to ABA. Previously we reported that the second and fourth positions in the VxGPhiL motif of PP2Cs from Oryza sativa are critical in the interaction of PP2Cs with PYL/RCARs. Considering substantial effects of the VxGPhiL motif on ABA signaling outputs, further comprehensive characterization of residues in the second and fourth positions are required. Here we surveyed the second and fourth positions of the VxGPhiL motif by combination of biochemical, structural and physiological analyses. We found that the fourth position of the VxGPhiL motif, highly conserved to small hydrophobic residues, was a key determinant of the OsPP2C50:OsPYL/RCAR interactions across subfamilies. Large hydrophobic or any hydrophilic residues in the fourth position abrogated ABA responsiveness. Analysis of crystal structures of OsPP2C50 mutants, S265L/I267V ("LV"), I267L ("SL") and I267W ("SW"), in complex with ABA and OsPYL/RCAR3, along with energy calculation of the complexes, uncovered that a bulky hydrophobic residue in the fourth position of the VxGPhiL motif pushed away side chains of nearby residues, conferring side-chain rotameric energy stress. Hydrophilic residues in this position imposed solvation energy stress to the PP2C:PYL/RCAR complex. Germination and gene expression analyses corroborated that OsPP2C50 AS and AK mutants modulated ABA responsiveness in Arabidopsis. Our results suggest that ABA responsiveness could be fine-tuned by the fourth position of the VxGPhiL motif on PP2Cs. KEY MESSAGE: We comprehensively surveyed the VxGPhiL motif to find that the fourth position, highly conserved to small hydrophobic residues, was critical in regulating ABA responsiveness.
Comprehensive survey of the VxGPhiL motif of PP2Cs from Oryza sativa reveals the critical role of the fourth position in regulation of ABA responsiveness.,Han S, Lee JY, Lee Y, Kim TH, Lee S Plant Mol Biol. 2019 Nov;101(4-5):455-469. doi: 10.1007/s11103-019-00916-9. Epub , 2019 Sep 20. PMID:31541388[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ He Y, Hao Q, Li W, Yan C, Yan N, Yin P. Identification and characterization of ABA receptors in Oryza sativa. PLoS One. 2014 Apr 17;9(4):e95246. doi: 10.1371/journal.pone.0095246. eCollection, 2014. PMID:24743650 doi:http://dx.doi.org/10.1371/journal.pone.0095246
- ↑ Tian X, Wang Z, Li X, Lv T, Liu H, Wang L, Niu H, Bu Q. Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice. Rice (N Y). 2015 Dec;8(1):28. doi: 10.1186/s12284-015-0061-6. Epub 2015 Sep 11. PMID:26362328 doi:http://dx.doi.org/10.1186/s12284-015-0061-6
- ↑ Han S, Min MK, Lee SY, Lim CW, Bhatnagar N, Lee Y, Shin D, Chung KY, Lee SC, Kim BG, Lee S. Modulation of ABA signaling by altering VxGPhiL motif of PP2Cs in Oryza sativa. Mol Plant. 2017 Aug 18. pii: S1674-2052(17)30233-2. doi:, 10.1016/j.molp.2017.08.003. PMID:28827170 doi:http://dx.doi.org/10.1016/j.molp.2017.08.003
- ↑ Han S, Lee JY, Lee Y, Kim TH, Lee S. Comprehensive survey of the VxGPhiL motif of PP2Cs from Oryza sativa reveals the critical role of the fourth position in regulation of ABA responsiveness. Plant Mol Biol. 2019 Nov;101(4-5):455-469. doi: 10.1007/s11103-019-00916-9. Epub , 2019 Sep 20. PMID:31541388 doi:http://dx.doi.org/10.1007/s11103-019-00916-9
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