Structural highlights
Function
[MURJ_THEAB] Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.[HAMAP-Rule:MF_02078][1]
Publication Abstract from PubMed
The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors.
Visualizing conformation transitions of the Lipid II flippase MurJ.,Kuk ACY, Hao A, Guan Z, Lee SY Nat Commun. 2019 Apr 15;10(1):1736. doi: 10.1038/s41467-019-09658-0. PMID:30988294[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kuk AC, Mashalidis EH, Lee SY. Crystal structure of the MOP flippase MurJ in an inward-facing conformation. Nat Struct Mol Biol. 2016 Dec 26. doi: 10.1038/nsmb.3346. PMID:28024149 doi:http://dx.doi.org/10.1038/nsmb.3346
- ↑ Kuk ACY, Hao A, Guan Z, Lee SY. Visualizing conformation transitions of the Lipid II flippase MurJ. Nat Commun. 2019 Apr 15;10(1):1736. doi: 10.1038/s41467-019-09658-0. PMID:30988294 doi:http://dx.doi.org/10.1038/s41467-019-09658-0
