Structural highlights
Publication Abstract from PubMed
A nonpolar aromatic nucleoside derivative based on 2,4-difluorotoluene (F), a non-hydrogen bonding shape analog of thymidine, was recently shown to be replicated against adenine with high efficiency and fidelity. This led to the suggestion that geometric matching, potentially even in the absence of hydrogen bonding between bases in a pair, may be sufficient to direct nucleotide selection during replication. We have examined the solution structure of the F-A pair in the context of a 12 base pair DNA duplex. We find that, despite the destabilization caused by this analog, the F-A pair very closely resembles that of a T x A pair in the same context. This lends support to the importance of shape matching in replication.
Solution structure of a DNA duplex containing a replicable difluorotoluene-adenine pair.,Guckian KM, Krugh TR, Kool ET Nat Struct Biol. 1998 Nov;5(11):954-9. PMID:9808039[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guckian KM, Krugh TR, Kool ET. Solution structure of a DNA duplex containing a replicable difluorotoluene-adenine pair. Nat Struct Biol. 1998 Nov;5(11):954-9. PMID:9808039 doi:10.1038/2930
