6p2r

Structural highlights

Function

[PMT1_YEAST] Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT2 and more rarely with PMT3 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. The PMT1-PMT2 complex participates in oxidative protein folding, ER-associated protein degradation (ERAD), as well as ER export. Required for incorporation of proteins in the cell wall.^{[1] [2] [3] [4] [5]} [PMT2_YEAST] Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Forms a heterodimeric complex with PMT2 and more rarely with PMT5 to transfer mannose from Dol-P-mannose to Ser or Thr residues on proteins. The PMT1-PMT2 complex participates in oxidative protein folding, ER-associated protein degradation (ERAD), as well as ER export.^{[6] [7] [8] [9]}

References

1. ↑ Girrbach V, Zeller T, Priesmeier M, Strahl-Bolsinger S. Structure-function analysis of the dolichyl phosphate-mannose: protein O-mannosyltransferase ScPmt1p. J Biol Chem. 2000 Jun 23;275(25):19288-96. doi: 10.1074/jbc.M001771200. PMID:10764776 doi:http://dx.doi.org/10.1074/jbc.M001771200
2. ↑ Hirayama H, Fujita M, Yoko-o T, Jigami Y. O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae. J Biochem. 2008 Apr;143(4):555-67. doi: 10.1093/jb/mvm249. Epub 2008 Jan 7. PMID:18182384 doi:http://dx.doi.org/10.1093/jb/mvm249
3. ↑ Strahl-Bolsinger S, Immervoll T, Deutzmann R, Tanner W. PMT1, the gene for a key enzyme of protein O-glycosylation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8164-8. PMID:8367478
4. ↑ Gentzsch M, Immervoll T, Tanner W. Protein O-glycosylation in Saccharomyces cerevisiae: the protein O-mannosyltransferases Pmt1p and Pmt2p function as heterodimer. FEBS Lett. 1995 Dec 18;377(2):128-30. doi: 10.1016/0014-5793(95)01324-5. PMID:8543034 doi:http://dx.doi.org/10.1016/0014-5793(95)01324-5
5. ↑ Bourdineaud JP, van der Vaart JM, Donzeau M, de Sampaio G, Verrips CT, Lauquin GJ. Pmt1 mannosyl transferase is involved in cell wall incorporation of several proteins in Saccharomyces cerevisiae. Mol Microbiol. 1998 Jan;27(1):85-98. PMID:9466258
6. ↑ Nakatsukasa K, Okada S, Umebayashi K, Fukuda R, Nishikawa S, Endo T. Roles of O-mannosylation of aberrant proteins in reduction of the load for endoplasmic reticulum chaperones in yeast. J Biol Chem. 2004 Nov 26;279(48):49762-72. doi: 10.1074/jbc.M403234200. Epub 2004, Sep 17. PMID:15377669 doi:http://dx.doi.org/10.1074/jbc.M403234200
7. ↑ Hirayama H, Fujita M, Yoko-o T, Jigami Y. O-mannosylation is required for degradation of the endoplasmic reticulum-associated degradation substrate Gas1*p via the ubiquitin/proteasome pathway in Saccharomyces cerevisiae. J Biochem. 2008 Apr;143(4):555-67. doi: 10.1093/jb/mvm249. Epub 2008 Jan 7. PMID:18182384 doi:http://dx.doi.org/10.1093/jb/mvm249
8. ↑ Goder V, Melero A. Protein O-mannosyltransferases participate in ER protein quality control. J Cell Sci. 2011 Jan 1;124(Pt 1):144-53. doi: 10.1242/jcs.072181. Epub 2010 Dec, 8. PMID:21147851 doi:http://dx.doi.org/10.1242/jcs.072181
9. ↑ Gentzsch M, Immervoll T, Tanner W. Protein O-glycosylation in Saccharomyces cerevisiae: the protein O-mannosyltransferases Pmt1p and Pmt2p function as heterodimer. FEBS Lett. 1995 Dec 18;377(2):128-30. doi: 10.1016/0014-5793(95)01324-5. PMID:8543034 doi:http://dx.doi.org/10.1016/0014-5793(95)01324-5