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1hbi

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Revision as of 10:59, 21 February 2008 by OCA (Talk | contribs)
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1hbi, resolution 1.7Å

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CRYSTAL STRUCTURE OF OXYGENATED SCAPHARCA DIMERIC HEMOGLOBIN AT 1.7 ANGSTROMS RESOLUTION

Overview

The crystal structure of the cooperative dimeric hemoglobin from the blood clam Scapharca inaequivalvis has been determined in the oxygenated state and refined to an R-factor of 0.157 at 1.7-A resolution. The structure is very similar to the carbon monoxide-liganded form with subtle differences in ligand binding geometry. Oxygen binds to the heme iron in a bent conformation with Fe-O-O angles of 135 degrees and 150 degrees for the two subunits. These observed angles are lower than the equivalent angles in the carbon monoxide-liganded form and intermediate between the angles observed in structures of oxygenated sperm whale myoglobin and oxygenated human hemoglobin. This third high resolution structure of Scapharca dimeric hemoglobin permits a detailed analysis of the water structure in the highly hydrated interface between subunits.

About this Structure

1HBI is a Single protein structure of sequence from Scapharca inaequivalvis with and as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of oxygenated Scapharca dimeric hemoglobin at 1.7-A resolution., Condon PJ, Royer WE Jr, J Biol Chem. 1994 Oct 14;269(41):25259-67. PMID:7929217

Page seeded by OCA on Thu Feb 21 12:59:29 2008

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