2buf

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spinqualitylabelsall models 2buf, resolution 2.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ARGININE FEED-BACK INHIBITABLE ACETYLGLUTAMATE KINASE

Overview

N-Acetylglutamate kinase (NAGK) catalyses the second step in the route of, arginine biosynthesis. In many organisms this enzyme is inhibited by the, final product of the route, arginine, and thus plays a central regulatory, role. In addition, in photosynthetic organisms NAGK is the target of the, nitrogen-signalling protein PII. The 3-D structure of homodimeric, arginine-insensitive, Escherichia coli NAGK, clarified substrate binding, and catalysis but shed no light on arginine inhibition of NAGK. We now, shed light on arginine inhibition by determining the crystal structures, at 2.75 A and 2.95 A resolution, of arginine-complexed Thermotoga maritima, and arginine-free Pseudomonas aeruginosa NAGKs, respectively. Both enzymes, are highly similar ring-like hexamers having a central orifice ... [(full description)]

About this Structure

2BUF is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with MG, CL, NLG and ADP as [ligands]. This structure superseeds the now removed PDB entry 1UVD. Active as [Acetylglutamate kinase], with EC number [2.7.2.8]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural bases of feed-back control of arginine biosynthesis, revealed by the structures of two hexameric N-acetylglutamate kinases, from Thermotoga maritima and Pseudomonas aeruginosa., Ramon-Maiques S, Fernandez-Murga ML, Gil-Ortiz F, Vagin A, Fita I, Rubio V, J Mol Biol. 2006 Feb 24;356(3):695-713. Epub 2005 Dec 12. PMID:16376937

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