Structural highlights
Publication Abstract from PubMed
We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing employing brominated oligonucleotides. The DNA-drug complex has a unique structure that combines alkylation (at the N7 position of a guanine), intercalation (on the 3'-side of the alkylated guanine), and base flip-out. An antibiotic-induced flipping-out of a single, nonterminal nucleobase from a DNA duplex was observed for the first time in a crystal structure.
Crystal structure of trioxacarcin A covalently bound to DNA.,Pfoh R, Laatsch H, Sheldrick GM Nucleic Acids Res. 2008 May 3;. PMID:18453630[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pfoh R, Laatsch H, Sheldrick GM. Crystal structure of trioxacarcin A covalently bound to DNA. Nucleic Acids Res. 2008 May 3;. PMID:18453630 doi:http://dx.doi.org/gkn245
