| Structural highlights
7a20 is a 4 chain structure with sequence from "bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , |
| Gene: | trpB, A2O69_02700, A3104_03435, A3111_06740, A3122_00260, A3146_03375, A3R41_00170, A3S30_00170, A3T21_03460, A3T81_00170, A3U32_00155, A3V03_00170, A3V89_11050, A3W57_00170, A3W86_01970, A3X15_04680, A3X55_12370, A3Y76_02015, A3Z96_03135, A4J27_03520, A4N07_00170, A4O05_04695, A4O41_00155, A4R48_02630, A6D61_00175, A9C21_03185, A9T39_18110, A9U46_07415, A9U64_18370, AAA76_00155, AAB27_09275, AAB39_14815, AAB79_17610, AAC08_07460, AAC35_00170, AAC42_18415, AAC44_05110, AAC95_06070, AAC98_13030, AAP89_02045, AAQ24_18000, AB424_02945, ABO94_19170, ABP11_12175, ABP54_18795, ABP87_03855, ABQ69_14880, ADQ28_02550, ADQ45_00245, AE787_02700, AF480_03975, AF488_06015, AF489_02660, AF497_08120, AGC55_13735, AGM99_16800, AGQ32_20575, AH984_18395, AHN93_02395, AIC76_02215, AKH62_04245, AKH68_02480, AKI16_02910, AL144_16660, AL151_17300, AL166_18250, AL168_15625, AL170_16750, AL174_09880, AL184_20200, APP08_15530, APY91_03765, AQ530_01180, AS118_02480, AT354_12415, AU613_22485, AU805_00170, AU830_08700, AU839_18255, AU951_15155, AU965_03080, AVA38_22260, AVB77_10100, AVB94_04700, AVC05_15215, AVD75_00170, AVD94_08015, AVG17_00170, AVL02_12430, AVL16_02480, AVM19_00180, AWT30_03270, AXM10_01880, AXM23_01435, AXR84_21850, AXU58_18720, AXX99_00245, B1265_17730, B1398_17380, B1642_03090, B1B86_18925, B1B89_03525, B1I91_02730, B1P38_14650, B1Q82_16845, B2E31_01130, B4V59_01130, B4W90_02480, B5A40_16840, B6362_18050, B6G98_13735, B6M25_03790, B6M43_05335, B7071_04185, B7890_00180, B7J30_16900, B7Q27_19125, B8Y16_01430, B8Y36_00160, B8Z46_02480, B9653_00860, B9C61_00155, B9C71_05005, B9C90_02615, B9C91_02585, B9C96_00170, B9M14_03090, B9O84_04980, B9U29_02980, BBQ66_00245, BEL47_09240, BFE55_13570, BFE61_16680, BGP52_00170, BIC00_16200, BIC01_10585, BIC03_16630, BIC13_14200, BJP89_17200, BK110_16905, BKM50_00480, BLB03_15985, BMG85_08680, BMS46_07360, BMU56_00245, BSC80_03850, BSD55_03835, BZ203_00610, BZ210_00245, BZG47_02660, BZN20_03410, BZZ88_16730, C4E88_03765, C5W43_02660, CA117_01340, CB102_03625, CB119_10850, CB161_01685, CB198_15695, CB380_00170, CB383_15125, CB416_01745, CB530_00170, CB535_01780, CB570_06135, CB646_05195, CB657_17405, CBH20_00180, CBM40_00170, CBM67_00170, CBM76_00170, CBN77_01665, CBO42_03390, CBR08_18065, CBU32_02810, CBZ90_16175, CC339_09505, CC403_14745, CC453_16030, CC594_08915, CC652_03225, CC725_00155, CC886_03030, CC918_08540, CC944_16235, CC971_02330, CCF93_01200, CCP17_15505, CCW27_18540, CD48_01255, CD977_00555, CDJ75_00185, CDT37_16835, CDZ72_11630, CE355_01000, CE615_00490, CE70_00965, CE806_19055, CE87_00490, CE896_13665, CEC46_02425, CEC56_00180, CED07_00245, CEQ70_19140, CER60_14710, CER78_02650, CET98_00490, CEY64_04070, CFB16_19760, CFB28_02660, CFE76_03600, CFE79_19515, CFF58_02660, CFF59_02520, CFJ48_19425, CGG73_00635, CHN22_02480, CI444_00490, CIX60_05915, CJC42_00255, CPR79_00170, CPS79_00175, CPX68_00155, CQE35_02195, CQG18_02715, CQO33_05135, CR370_00800, CR439_06560, CRB02_00255, CSG22_09800, CTJ81_13375, CVR97_02985, D3147_03410, D3174_02070, D3F31_17945, D3T68_17470, D3Y48_10485, D4361_01920, D4369_03495, D4380_02480, D4387_05640, D4422_03590, D4E62_00155, D4E68_07210, D4E74_05090, D4X64_05245, D4X79_00160, D4Y62_02080, D5823_04020, D5949_01705, D5B48_00170, D5C67_05340, D5C71_03585, D5N86_03370, D5N95_03780, D5O82_00170, D5P17_00175, D5X47_00155, D5Y28_02000, D6360_05135, D6367_06240, D6371_00170, D6373_01600, D6395_18520, D6421_01600, D6422_13010, D6J79_06100, D6K10_14635, D6P67_05630, D6Q64_00170, D6S43_00170, D6T00_04265, D6T40_02405, D7F20_01770, D7H43_22440, D7N92_00170, D7O44_03140, D8Q90_00155, D8S24_03730, D9O84_00170, DD95_04150, DJ388_09100, DJ702_07170, DK061_00170, DK631_13470, DK641_13525, DK642_09865, DK689_04170, DK696_00155, DK698_07515, DKJ10_06070, DKR95_00180, DKS55_13075, DKU45_00170, DKU57_01740, DKU80_09490, DLB14_00170, DLB57_04220, DLB93_03785, DLR28_07055, DM322_07705, DMO92_04585, DMV40_19520, DMZ93_09325, DN165_02735, DN204_04805, DN359_09815, DN904_05235, DNB97_02885, DNL62_13150, DNM27_00170, DNM63_00170, DNU59_01735, DNV08_02465, DNV12_06770, DNV30_02640, DNY92_05160, DNZ37_13570, DO350_14195, DO533_00155, DO585_06995, DO640_03045, DO698_02865, DO766_01995, DO828_00170, DO960_02190, DOC60_08280, DOH72_01755, DOI32_03380, DOI53_01970, DOI92_02220, DOJ39_00170, DOJ91_01000, DOQ54_00165, DOQ88_05395, DOR12_01820, DOV43_07020, DOW25_01950, DP680_05140, DPB42_18065, DPB45_01840, DPB48_03660, DPB57_00475, DPD91_03685, DPD95_01735, DPD99_16170, DPF41_04665, DPF68_00170, DPK32_00175, DPK79_00170, DPL02_00250, DPP94_16895, DPP97_00170, DPS76_03540, DPT18_00170, DPU20_00155, DPY58_03130, DQ848_22185, DQ947_07375, DQ951_00170, DQC39_04660, DQC52_04025, DQD03_00170, DQD22_02705, DQE64_05330, DQE65_07780, DQK42_00170, DQQ98_01490, DQR10_06600, DQR44_00160, DQR61_14690, DQS14_15280, DQY10_00180, DQZ46_17885, DQZ56_00170, DR982_04285, DRL45_00170, DRM14_00170, DRM16_05700, DRR75_11000, DRT38_04270, DRT61_09475, DRT65_11180, DRV05_00155, DRW84_00165, DRX66_18270, DS270_00165, DS296_18555, DS451_00165, DS453_03515, DS521_00170, DS619_01755, DSF69_00170, DSF94_07470, DSG41_00170, DSM38_03600, DSN15_07080, DSR36_00155, DTE73_04570, DTF68_04455, DTG22_18835, DTG27_10395, DTW13_02015, DTW14_04725, DTW26_04255, DU071_15080, DU223_01840, DU657_05885, DU821_03575, DU879_04030, DU924_01710, DUQ28_05210, DUQ56_02020, DUQ92_13430, DUR36_02795, DUV75_02780, DUW10_00170, DUW48_00170, DVF14_00170, DVG01_03400, DVZ53_00970, DWU22_05270, DY580_18360, DYM27_15970, DYS82_04405, DZG11_04025, E0595_03335, E0935_04475, E0989_02690, E0M34_03830, E0U75_00175, E0V94_01725, E1A11_00175, E1A20_21780, E5196_00320, E6W45_19450, EBB93_00170, EBC01_00255, EBD14_01915, EBD99_05490, EBK21_00175, EBL31_00495, EBO41_01950, EBP31_03920, EC404_00175, EC52_01130, ECA50_02910, ECC89_03735, ED424_01900, ED467_02975, EDL18_05830, EEK73_02630, EEQ30_08395, EER35_12065, EGL32_03690, EGN81_18590, EGU67_04975, EGU98_07515, EHB09_01390, EHB24_02705, EHB55_00170, EHC98_05585, EIE48_00175, EIW53_01745, EJI18_00160, EJO08_03330, EJO98_00570, EKA25_00505, EL822_00180, ELO47_03135, ELR28_01920, ELS01_00160, EM832_04945, EM840_00170, EMN66_03645, EMY79_01620, EO190_03995, EP115_03030, EP446_10225, EPB30_00175, EPH81_02045, EQG93_01820, EQG94_00170, ERM04_00175, EU306_00170, EU349_00160, EU873_02910, EUB95_14530, EUQ56_04030, EUQ65_02370, EUQ74_06795, EUS13_04115, EVY71_00185, EW905_13425, EWB18_18060, EWE52_00160, EWJ47_00255, EWZ09_03890, EXA47_00255, EXB41_01730, EYA29_11490, EYJ91_04475, F0D96_02430, F2O93_00610, F2P00_03525, F3Q46_10715, F3Q58_08085, F3Q59_08005, F3Q88_02710, F3Q97_02935, F3R12_00175, F3R61_03875, F3R63_00165, F7773_04555, F9G02_00155, F9O44_16550, FE758_04150, FEM52_02625, FGZ46_01680, FJM64_00170, FL833_00155, FQC24_03095, FQD13_06720, FQX78_06105, GCH31_00155, GCQ81_00170, GCZ80_03085, GEZ01_04020, GW08_03175, JO10_00490, KP44_16045, LZ63_02660, NG02_04175, NG06_16730, NG18_14010, NU83_02660, QA89_14465, QB40_00170, QD15_05225, R035_09175, RJ78_16500, SAMEA4398682_02247, SE14_01810, Y934_00965, YG50_13195, YR17_00170, YT65_03520, Z700_00170, ZB89_05455, ZC54_00170, ZT09_07610, ZT28_00165, ZT74_16550, ZT89_11225, ZU86_12135, ZU92_17280, ZV06_15535, ZV17_18195, ZV33_00165, ZV34_00170, ZV38_00170, ZV58_05880, ZV70_10435, ZV76_15965, ZV78_00165, ZV90_13185, ZW74_18840, ZX03_08400, ZY23_01880, ZY27_00170, ZY40_08870, ZY51_17025, ZZ18_11980, ZZ43_16750, ZZ77_00155, ZZ79_17015 ("Bacillus typhimurium" Loeffler 1892) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[A0A0D6FWC1_SALTM] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.[HAMAP-Rule:MF_00131][SAAS:SAAS01111173]
Publication Abstract from PubMed
Light-regulation of drug molecules has gained growing interest in the fields of biochemical and pharmacological research in recent years. In addition, a serious need for novel molecular targets of antibiotics has emerged presently. Here, we present the development of a photo-controllable, azobenzene-based antibiotic precursor towards tryptophan synthase (TS), an essential metabolic multienzyme complex in bacteria. The compound exhibited a medium-strong inhibition of TS in its E configuration, and a 5-fold lower inhibition strength in its Z configuration. Using a combination of biochemical, crystallographic, and computational analyses we characterized the inhibition mode of this compound. Remarkably, binding of the inhibitor to a hitherto not considered cavity causes an unproductive conformation of TS leading to non-competitive inhibition of tryptophan production. In conclusion, we created a promising lead compound for the combat of bacterial diseases, which targets an essential metabolic enzyme, and whose inhibition strength can be controlled with light.
Towards Photochromic Azobenzene-Based Inhibitors for Tryptophan Synthase.,Simeth NA, Kinateder T, Rajendran C, Nazet J, Merkl R, Sterner R, Konig B, Kneuttinger AC Chemistry. 2020 Oct 20. doi: 10.1002/chem.202004061. PMID:33078454[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Simeth NA, Kinateder T, Rajendran C, Nazet J, Merkl R, Sterner R, Konig B, Kneuttinger AC. Towards Photochromic Azobenzene-Based Inhibitors for Tryptophan Synthase. Chemistry. 2020 Oct 20. doi: 10.1002/chem.202004061. PMID:33078454 doi:http://dx.doi.org/10.1002/chem.202004061
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