Structural highlights
Function
BCSE_ECOLI Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP), the ability to bind c-di-GMP is important for its function (PubMed:24942809).[1]
Publication Abstract from PubMed
Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation.
Architecture and regulation of an enterobacterial cellulose secretion system.,Abidi W, Zouhir S, Caleechurn M, Roche S, Krasteva PV Sci Adv. 2021 Jan 27;7(5). pii: 7/5/eabd8049. doi: 10.1126/sciadv.abd8049. Print , 2021 Jan. PMID:33563593[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fang X, Ahmad I, Blanka A, Schottkowski M, Cimdins A, Galperin MY, Romling U, Gomelsky M. GIL, a new c-di-GMP-binding protein domain involved in regulation of cellulose synthesis in enterobacteria. Mol Microbiol. 2014 Aug;93(3):439-52. doi: 10.1111/mmi.12672. Epub 2014 Jul 1. PMID:24942809 doi:http://dx.doi.org/10.1111/mmi.12672
- ↑ Abidi W, Zouhir S, Caleechurn M, Roche S, Krasteva PV. Architecture and regulation of an enterobacterial cellulose secretion system. Sci Adv. 2021 Jan 27;7(5):eabd8049. PMID:33563593 doi:10.1126/sciadv.abd8049
