1ev7
From Proteopedia
CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI
Structural highlights
FunctionT2N1_LENAE An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.[1] [2] [3] Publication Abstract from PubMedNAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes. Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase.,Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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