Introduction
ACAT is a [tetramer] composed of a dimer of dimers.
Mechanism
The mechanism of the acyltransferase reaction occurs in the catalytic site one of the monomers in the dimer of ACAT1. The T tunnel and and C tunnel converge to the same space to allow the proper orientation of the Acyl CoA and the incoming cholesterol from the transmembrane. The Acyl CoA is oriented in a way to allow the His460 to act as a base catalyst to begin the reaction by deprotonation of the cholesterol which allows it to attack the carbonyl carbon which breaks the sulfur carbonyl bond (figure X). This mechanism produced Acyl-CoASH and cholesteryl ester. The Acyl-CcASH leaves through the C tunnel to the cytosol.
Crystal Structure of the Entamoeba histolytica RNA lariat debranching enzyme. [1]
Function
ACAT article [2]
SOAT Article [3]
Disease
Relevance
Structural highlights
The that constitute the T tunnel are critical to the activity of ACAT1.
