7d2y
From Proteopedia
complex of two RRM domains
Structural highlights
FunctionTOFU6_CAEEL Component of the pid-1 and tost-1 variants of the PETISCO complexes, which have roles in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-RNAs) and embryogenesis, respectively (PubMed:31147388, PubMed:31216475). Promotes the biogenesis of 21U-RNAs (PubMed:31216475). Required for chromosome segregation and cell division in early embryos (PubMed:17171368, PubMed:31216475). May have a role in DNA replication (PubMed:17171368).[1] [2] [3] [4] Publication Abstract from PubMedBy incorporating two mutually exclusive factors, PID-1 and TOST-1, C. elegans PICS complex plays important roles in piRNA biogenesis, chromosome segregation and cell division. We firstly map the interaction network between PICS subunits, then uncover the mechanisms underlying the interactions between PICS subunits by solving several complex structures, including those of TOFU-6/PICS-1, ERH-2/PICS-1, and ERH-2/TOST-1. Our biochemical experiment also demonstrates that PICS exists as an octamer consisting of two copies of each subunit. Combining structural analyses with mutagenesis experiments, we identify interfacial residues of PICS subunits that are critical for maintaining intact PICS complex in vitro. Furthermore, using genetics, cell biology and imaging experiments, we find that those mutants impairing the in vitro interaction network within PICS, also lead to dysfunction of PICS in vivo, including mislocalization of PICS, and reduced levels of piRNAs or aberrant chromosome segregation and cell division. Therefore, our work provides structural insights into understanding the PICS-mediated piRNA biogenesis and cell division. Molecular basis for PICS-mediated piRNA biogenesis and cell division.,Wang X, Zeng C, Liao S, Zhu Z, Zhang J, Tu X, Yao X, Feng X, Guang S, Xu C Nat Commun. 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7. PMID:34552083[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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