This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1h6h
From Proteopedia
|
STRUCTURE OF THE PX DOMAIN FROM P40PHOX BOUND TO PHOSPHATIDYLINOSITOL 3-PHOSPHATE
Overview
More than 50 human proteins with a wide range of functions have a 120, residue phosphoinositide binding module known as the PX domain. The 1.7 A, X-ray crystal structure of the PX domain from the p40(phox) subunit of, NADPH oxidase bound to PtdIns(3)P shows that the PX domain embraces the, 3-phosphate on one side of a water-filled, positively charged pocket and, reveals how 3-phosphoinositide specificity is achieved. A chronic, granulomatous disease (CGD)-associated mutation in the p47(phox) PX domain, that abrogates PtdIns(3)P binding maps to a conserved Arg that does not, directly interact with the phosphoinositide but instead appears to, stabilize a critical lipid binding loop. The SH3 domain present in the, full-length protein does not affect soluble PtdIns(3)P binding to the, p40(phox) PX domain.
About this Structure
1H6H is a Single protein structure of sequence from Homo sapiens with PIB and GOL as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate., Bravo J, Karathanassis D, Pacold CM, Pacold ME, Ellson CD, Anderson KE, Butler PJ, Lavenir I, Perisic O, Hawkins PT, Stephens L, Williams RL, Mol Cell. 2001 Oct;8(4):829-39. PMID:11684018
Page seeded by OCA on Tue Dec 18 16:03:58 2007
Categories: Homo sapiens | Single protein | Bravo, J. | Karathanassis, D. | Pacold, M. | Perisic, O. | Williams, R.L. | GOL | PIB | Px domain
