1hdr

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Image:1hdr.gif

1hdr, resolution 2.5Å

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THE CRYSTALLOGRAPHIC STRUCTURE OF A HUMAN DIHYDROPTERIDINE REDUCTASE NADH BINARY COMPLEX EXPRESSED IN ESCHERICHIA COLI BY A CDNA CONSTRUCTED FROM ITS RAT HOMOLOGUE

Contents

Overview

A human dihydropteridine reductase (EC 1.6.99.10) has been created from a rat cDNA clone by a single five-oligonucleotide mutagenesis reaction and expressed in good yield in Escherichia coli. The enzyme has been purified to homogeneity, and kinetic identity to the naturally occurring enzyme has been proven. Crystallization has also been achieved, and the crystal structure was solved using 2.5 A data that was refined to an R value of 16.9%. The structure described in this report represents the first complete structural characterization of this important human enzyme.

Disease

Known diseases associated with this structure: Phenylketonuria due to dihydropteridine reductase deficiency OMIM:[261630]

About this Structure

1HDR is a Single protein structure of sequence from Homo sapiens with as ligand. Active as 6,7-dihydropteridine reductase, with EC number 1.5.1.34 Full crystallographic information is available from OCA.

Reference

The crystallographic structure of a human dihydropteridine reductase NADH binary complex expressed in Escherichia coli by a cDNA constructed from its rat homologue., Su Y, Varughese KI, Xuong NH, Bray TL, Roche DJ, Whiteley JM, J Biol Chem. 1993 Dec 25;268(36):26836-41. PMID:8262916

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