7np8
From Proteopedia
Crystal structure of the Coenzyme F420-dependent sulfite reductase from Methanocaldococcus jannaschii at 2.3-A resolution
Structural highlights
FunctionFSR_METJA Catalyzes the reduction of sulfite to sulfide using reduced F420 as the electron source. Involved in sulfite detoxification and assimilation. Cannot use NADH or NADPH.[1] [2] Publication Abstract from PubMedMethanogenic archaea are main actors in the carbon cycle but are sensitive to reactive sulfite. Some methanogens use a sulfite detoxification system that combines an F(420)H(2)-oxidase with a sulfite reductase, both of which are proposed precursors of modern enzymes. Here, we present snapshots of this coupled system, named coenzyme F(420)-dependent sulfite reductase (Group I Fsr), obtained from two marine methanogens. Fsr organizes as a homotetramer, harboring an intertwined six-[4Fe-4S] cluster relay characterized by spectroscopy. The wire, spanning 5.4 nm, electronically connects the flavin to the siroheme center. Despite a structural architecture similar to dissimilatory sulfite reductases, Fsr shows a siroheme coordination and a reaction mechanism identical to assimilatory sulfite reductases. Accordingly, the reaction of Fsr is unidirectional, reducing sulfite or nitrite with F(420)H(2). Our results provide structural insights into this unique fusion, in which a primitive sulfite reductase turns a poison into an elementary block of life. Structures of the sulfite detoxifying F(420)-dependent enzyme from Methanococcales.,Jespersen M, Pierik AJ, Wagner T Nat Chem Biol. 2023 Jan 19. doi: 10.1038/s41589-022-01232-y. PMID:36658338[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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