Structural highlights
Function
EX7S_ECOLI Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides. It can degrade 3' or 5' ss regions extending from the termini of duplex DNA molecules and displaced ss regions. It can also excise thymine dimers in vitro (PubMed:4602029, PubMed:4602030, PubMed:22718974) (Probable). Required for production of the mature 5'-end of retron Ec78 or Ec83 msDNA. When in excess of the large subunit, counteracts the large subunit's toxicity (PubMed:26626352).[1] [2] [3] [4] [5]
References
- ↑ Poleszak K, Kaminska KH, Dunin-Horkawicz S, Lupas A, Skowronek KJ, Bujnicki JM. Delineation of structural domains and identification of functionally important residues in DNA repair enzyme exonuclease VII. Nucleic Acids Res. 2012 Sep;40(16):8163-74. PMID:22718974 doi:10.1093/nar/gks547
- ↑ Jung H, Liang J, Jung Y, Lim D. Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII. J Microbiol. 2015 Dec;53(12):820-8. PMID:26626352 doi:10.1007/s12275-015-5304-0
- ↑ Chase JW, Richardson CC. Exonuclease VII of Escherichia coli. Purification and properties. J Biol Chem. 1974 Jul 25;249(14):4545-52 PMID:4602029
- ↑ Chase JW, Richardson CC. Exonuclease VII of Escherichia coli. Mechanism of action. J Biol Chem. 1974 Jul 25;249(14):4553-61 PMID:4602030
- ↑ Vales LD, Rabin BA, Chase JW. Subunit structure of Escherichia coli exonuclease VII. J Biol Chem. 1982 Aug 10;257(15):8799-805 PMID:6284744
