2ggq

Structural highlights

Function

S1PNA_SULTO Bifunctional enzyme involved in the synthesis of UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-acetylgalactosamine (UDP-GalNAc). It has multiple amino-sugar-1-phosphate acetyltransferase activities, including glucosamine-1-phosphate (GlcN-1-P) acetyltransferase and galactosamine-1-phosphate (GalN-1-P) acetyltransferase activities, and multiple sugar-1-phosphate nucleotidylyltransferase activities, including N-acetylglucosamine-1-phosphate (GlcNAc-1-P) uridyltransferase and N-acetylgalactosamine-1-phosphate (GalNAc-1-P) uridyltransferase activities (PubMed:15598657, PubMed:20400541, PubMed:25567746). Also catalyzes the formation of dTDP-glucose from dTTP and glucose-1-phosphate (Glc-1-P), and the reverse reaction, which produces dTTP from dTDP-glucose and diphosphate (PubMed:15598657). Can also catalyze the formation of UDP-glucose from UTP and glucose-1-phosphate (PubMed:27864169, PubMed:30291121).^{[1] [2] [3] [4] [5]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Glucose-1-phosphate thymidylyltransferase 3D structures

References

1. ↑ Zhang Z, Tsujimura M, Akutsu J, Sasaki M, Tajima H, Kawarabayasi Y. Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain 7. J Biol Chem. 2005 Mar 11;280(10):9698-705. PMID:15598657 doi:10.1074/jbc.M411211200
2. ↑ Zhang Z, Akutsu J, Kawarabayasi Y. Identification of novel acetyltransferase activity on the thermostable protein ST0452 from Sulfolobus tokodaii strain 7. J Bacteriol. 2010 Jul;192(13):3287-93. PMID:20400541 doi:10.1128/JB.01683-09
3. ↑ Zhang Z, Shimizu Y, Kawarabayasi Y. Characterization of the amino acid residues mediating the unique amino-sugar-1-phosphate acetyltransferase activity of the archaeal ST0452 protein. Extremophiles. 2015 Mar;19(2):417-27. PMID:25567746 doi:10.1007/s00792-014-0727-9
4. ↑ Honda Y, Zang Q, Shimizu Y, Dadashipour M, Zhang Z, Kawarabayasi Y. Increasing the Thermostable Sugar-1-Phosphate Nucleotidylyltransferase Activities of the Archaeal ST0452 Protein through Site Saturation Mutagenesis of the 97th Amino Acid Position. Appl Environ Microbiol. 2017 Jan 17;83(3):e02291-16. PMID:27864169 doi:10.1128/AEM.02291-16
5. ↑ Honda Y, Nakano S, Ito S, Dadashipour M, Zhang Z, Kawarabayasi Y. Improvement of ST0452 GlcNAc-1-phosphate uridyltransferase activity by the cooperative effect of two single mutations identified through structure-based protein engineering. Appl Environ Microbiol. 2018 Oct 5. pii: AEM.02213-18. doi: 10.1128/AEM.02213-18. PMID:30291121 doi:http://dx.doi.org/10.1128/AEM.02213-18