2z5k
From Proteopedia
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Complex of Transportin 1 with TAP NLS
Overview
Transportin 1 (Trn1) is a transport receptor that transports substrates, from the cytoplasm to the nucleus through nuclear pore complexes by, recognizing nuclear localization signals (NLSs). Here we describe four, crystal structures of human Trn1 in a substrate-free form as well as in, the complex with three NLSs (hnRNP D, JKTBP, and TAP, respectively). Our, data have revealed that (1) Trn1 has two sites for binding NLSs, one with, high affinity (site A) and one with low affinity (site B), and NLS, interaction at site B controls overall binding affinity for Trn1; (2) Trn1, recognizes the NLSs at site A followed by conformational change at site B, to interact with the NLSs; and (3) a long flexible loop, characteristic of, Trn1, interacts with site B, thereby displacing transport substrate in the, nucleus. These studies provide deep understanding of substrate recognition, and dissociation by Trn1 in import pathways.
About this Structure
2Z5K is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for substrate recognition and dissociation by human transportin 1., Imasaki T, Shimizu T, Hashimoto H, Hidaka Y, Kose S, Imamoto N, Yamada M, Sato M, Mol Cell. 2007 Oct 12;28(1):57-67. PMID:17936704
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