This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1jj2
From Proteopedia
|
Fully Refined Crystal Structure of the Haloarcula marismortui Large Ribosomal Subunit at 2.4 Angstrom Resolution
Overview
Analysis of the Haloarcula marismortui large ribosomal subunit has revealed a common RNA structure that we call the kink-turn, or K-turn. The six K-turns in H.marismortui 23S rRNA superimpose with an r.m.s.d. of 1.7 A. There are two K-turns in the structure of Thermus thermophilus 16S rRNA, and the structures of U4 snRNA and L30e mRNA fragments form K-turns. The structure has a kink in the phosphodiester backbone that causes a sharp turn in the RNA helix. Its asymmetric internal loop is flanked by C-G base pairs on one side and sheared G-A base pairs on the other, with an A-minor interaction between these two helical stems. A derived consensus secondary structure for the K-turn includes 10 consensus nucleotides out of 15, and predicts its presence in the 5'-UTR of L10 mRNA, helix 78 in Escherichia coli 23S rRNA and human RNase MRP. Five K-turns in 23S rRNA interact with nine proteins. While the observed K-turns interact with proteins of unrelated structures in different ways, they interact with L7Ae and two homologous proteins in the same way.
About this Structure
1JJ2 is a Protein complex structure of sequences from Haloarcula marismortui with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
The kink-turn: a new RNA secondary structure motif., Klein DJ, Schmeing TM, Moore PB, Steitz TA, EMBO J. 2001 Aug 1;20(15):4214-21. PMID:11483524
Page seeded by OCA on Thu Feb 21 13:23:13 2008
Categories: Haloarcula marismortui | Protein complex | Klein, D J. | Moore, P B. | Schmeing, T M. | Steitz, T A. | CD | CL | K | MG | NA | Protein-protein | Protein-rna | Ribosome assembly | Rna-rna
