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1kq8
From Proteopedia
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Solution Structure of Winged Helix Protein HFH-1
Overview
The hepatocyte nuclear factor 3 (HNF-3)/fork head (fkh) family contains a large number of transcription factors that recognize divergent DNA sequences via a winged helix binding motif. HNF-3/fkh proteins show a broad profile of DNA sequence-specificity in which one DNA sequence can be recognized by more than one HNF-3/fkh protein and each individual HNF-3/fkh protein has several DNA binding sequences. In this study, heteronuclear NMR methods were used to study the structures of the DNA binding domain of a conserved winged helix protein HFH-1 and its DNA complexes. The structural comparison of winged helix proteins HFH-1 and Genesis and their DNA complexes indicates that even two highly conserved HNF-3 family members can adopt different local structures when they contact an identical DNA binding sequence, while one of these two HNF-3 proteins seems to adopt only slightly different structures on different DNA binding sites.
About this Structure
1KQ8 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure comparison of two conserved HNF-3/fkh proteins HFH-1 and genesis indicates the existence of folding differences in their complexes with a DNA binding sequence., Sheng W, Rance M, Liao X, Biochemistry. 2002 Mar 12;41(10):3286-93. PMID:11876636
Page seeded by OCA on Thu Feb 21 13:36:52 2008
Categories: Rattus norvegicus | Single protein | Liao, X. | Rance, M. | Sheng, W. | Hfh-1 | Nmr | Structure | Winged helix protein
