1omy
From Proteopedia
|
Crystal Structure of a Recombinant alpha-insect Toxin BmKaIT1 from the scorpion Buthus martensii Karsch
Overview
Alpha-insect scorpion toxins are a distinct group of scorpion neurotoxins for which no crystal structures are yet available. A novel alpha-insect toxin named BmKalphaIT1 from the scorpion Buthus martensii Karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKalphaIT1 (rBmKaIT1) was crystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 30.24 (1), b = 36.51 (3), c = 57.08 (2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKalphaIT1 molecule in the asymmetric unit.
About this Structure
1OMY is a Single protein structure of sequence from Mesobuthus martensii with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary crystallographic study of rBmKalphaIT1, a recombinant alpha-insect toxin from the scorpion Buthus martensii Karsch., Huang Y, Huang Q, Chen H, Tang Y, Miyake H, Kusunoki M, Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1635-6. Epub 2003, Aug 19. PMID:12925796
Page seeded by OCA on Thu Feb 21 14:19:34 2008
Categories: Mesobuthus martensii | Single protein | Chen, H. | Huang, Q. | Huang, Y. | Kusunoki, M. | Miyake, H. | Tang, Y. | ACY | CL | Alpha-insect toxin | Sodium channel
