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1s0u
From Proteopedia
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eIF2gamma apo
Overview
The x-ray structure of the gamma-subunit of the heterotrimeric translation initiation factor eIF2 has been determined to 2.4-A resolution. eIF2 is a GTPase that delivers the initiator Met-tRNA to the P site on the small ribosomal subunit during a rate-limiting initiation step in translation. The structure of eIF2gamma closely resembles that of EF1A.GTP, consisting of an N-terminal G domain followed by two beta-barrels arranged in a closed configuration with domain II packed against the G domain in the vicinity of the Switch regions. The G domain of eIF2gamma has an unusual zinc ribbon motif, not previously found in other GTPases. Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of eIF2gamma that bind the alpha-subunit and Met-tRNA(i)(Met), respectively. These structural, biochemical, and genetic results provide new insights into eIF2 ternary complex assembly.
About this Structure
1S0U is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
X-ray structure of translation initiation factor eIF2gamma: implications for tRNA and eIF2alpha binding., Roll-Mecak A, Alone P, Cao C, Dever TE, Burley SK, J Biol Chem. 2004 Mar 12;279(11):10634-42. Epub 2003 Dec 19. PMID:14688270
Page seeded by OCA on Thu Feb 21 14:56:42 2008
Categories: Methanocaldococcus jannaschii | Single protein | Alone, P. | Burley, S K. | Cao, C. | Dever, T E. | Roll-Mecak, A. | ZN | Ef-1a | Eif2 | Gtpase | Translation initiation | Trna
