1z7h
From Proteopedia
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2.3 Angstrom crystal structure of tetanus neurotoxin light chain
Overview
TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal structure of TeNT-LC, determined by combined molecular replacement and MAD phasing. As expected, the overall structure of TeNT-LC is similar to the other known CNT light chain structures, including a conserved thermolysin-like core inserted between structurally distinct amino- and carboxy-terminal regions. Differences between TeNT-LC and the other CNT light chains are mainly limited to surface features such as unique electrostatic potential profiles. An analysis of surface residue conservation reveals a pattern of relatively high variability matching the path of substrate binding around BoNT/A, possibly serving to accommodate the variations in different SNARE targets of the CNT group.
About this Structure
1Z7H is a Single protein structure of sequence from Clostridium tetani with as ligand. Active as Tentoxilysin, with EC number 3.4.24.68 Full crystallographic information is available from OCA.
Reference
2.3 A crystal structure of tetanus neurotoxin light chain., Breidenbach MA, Brunger AT, Biochemistry. 2005 May 24;44(20):7450-7. PMID:15895988
Page seeded by OCA on Thu Feb 21 16:12:48 2008
