1bq5

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Image:1bq5.gif

1bq5, resolution 2.05Å

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NITRITE REDUCTASE FROM ALCALIGENES XYLOSOXIDANS GIFU 1051

Overview

The crystal structure of the blue nitrite reductase from Alcaligenes, xylosoxidans GIFU 1051 (AxgNIR) has been determined at 2.05 A resolution., AxgNIR contains both type 1 and 2 Cu sites, the geometry of the former, being distorted tetrahedral. The superpositioning of the type 1 Cu sites, in the blue enzyme and a green nitrite reductase revealed that the, orientation of the Met150 side chain differed. The deviation of the, Sdelta(Met150) atom from the axial position of the NNS plane formed by two, Ndelta(His95 and His145) and one Sgamma(Cys136) atom caused the difference, in the colors of the enzymes, i.e. blue and green.

About this Structure

1BQ5 is a Single protein structure of sequence from Achromobacter xylosoxidans with CU as ligand. Structure known Active Sites: TP1 and TP2. Full crystallographic information is available from OCA.

Reference

Type 1 Cu structure of blue nitrite reductase from Alcaligenes xylosoxidans GIFU 1051 at 2.05 A resolution: comparison of blue and green nitrite reductases., Inoue T, Gotowda M, Deligeer, Kataoka K, Yamaguchi K, Suzuki S, Watanabe H, Gohow M, Kai Y, J Biochem (Tokyo). 1998 Nov;124(5):876-9. PMID:9792907

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