1h95
From Proteopedia
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SOLUTION STRUCTURE OF THE SINGLE-STRANDED DNA-BINDING COLD SHOCK DOMAIN (CSD) OF HUMAN Y-BOX PROTEIN 1 (YB1) DETERMINED BY NMR (10 LOWEST ENERGY STRUCTURES)
Overview
The human Y-box protein 1 (YB-1) is a member of the Y-box protein family, a class of proteins involved in transcriptional and translational, regulation of a wide range of genes. Here, we report the solution, structure of the cold-shock domain (CSD) of YB-1, which is thought to be, responsible for nucleic acid binding. It is the first structure solved of, a eukaryotic member of the cold-shock protein family and consists of a, closed five-stranded anti-parallel beta-barrel capped by a long flexible, loop. The structure of CSD is similar to the OB-fold and a comparison with, bacterial cold-shock proteins shows that its structural properties are, conserved from bacteria to man. Our data suggest the presence of a, DNA-binding site consisting of a patch of positively charged and aromatic, residues on the surface of the beta-barrel. Further, it is shown that CSD, which has a preference for binding single-stranded pyrimidine-rich, sequences, binds weakly and hardly specifically to DNA. Binding affinities, reported for intact YB-1 indicate that domains other than the CSD play a, role in DNA binding of YB-1.
About this Structure
1H95 is a Single protein structure of sequence from Homo sapiens. Structure known Active Site: DB. Full crystallographic information is available from OCA.
Reference
The solution structure and DNA-binding properties of the cold-shock domain of the human Y-box protein YB-1., Kloks CP, Spronk CA, Lasonder E, Hoffmann A, Vuister GW, Grzesiek S, Hilbers CW, J Mol Biol. 2002 Feb 15;316(2):317-26. PMID:11851341
Page seeded by OCA on Mon Nov 5 16:32:41 2007
