1iov
From Proteopedia
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COMPLEX OF D-ALA:D-ALA LIGASE WITH ADP AND A PHOSPHORYL PHOSPHONATE
Overview
The crystallographic structure of the D-alanine:D-alanine ligase of the, ddlB gene of Escherichia coli complexed with a, D-Ala-D-alpha-hydroxybutyrate phosphonate and the structure of the Y216F, mutant ligase complexed with a D-Ala-D-Ala phosphinate have been, determined to 2.2 and 1.9 A resolution, respectively, and refined to R, factors of 0.156 and 0.158. In each complex the inhibitor has reacted with, ATP to produce ADP and a tight-binding phosphorylated transition state, intermediate. Comparison of these two structures with the known crystal, structure of the phosphinate intermediate of the wild-type ligase shows no, major conformational changes, but B factors indicate differences in, mobility of loops covering the binding site. The weaker inhibition of the, Y216F mutant by both ... [(full description)]
About this Structure
1IOV is a [Single protein] structure of sequence from [Escherichia coli] with MG, ADP and POB as [ligands]. Active as [[1]], with EC number [6.3.2.4]. Full crystallographic information is available from [OCA].
Reference
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant., Fan C, Park IS, Walsh CT, Knox JR, Biochemistry. 1997 Mar 4;36(9):2531-8. PMID:9054558
Page seeded by OCA on Mon Oct 29 17:12:33 2007
Categories: Escherichia coli | Single protein | Fan, C. | Knox, J.R. | Moews, P.C. | ADP | MG | POB | Adp binding | Cell wall | Glycogen phosphorylase | Ligase | Peptidoglycan synthesis | Vancomycin
