Avidin

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Crystal Structure of Chicken Apo-Avidin, 1ave

Template:STRUCTURE 1ave 3D Structure of Avidin

Avidin (Av) is a protein found in egg white which binds biotin (vitamin B7) with high affinity. This property makes it a powerful tool in various protein pull-down assays. Similar proteins are strepavidin (StrAv) from the bacterium Streptomyces avidinii, Rhizavidin (RhiAv) from Rhizobium etli, Xenavidin from frog, and the avidin-related proteins AVR2 and AVR4. The N-acetylglucosamine sugars are shown as space filling objects in the image on the left.

1 Avidin
2 Strepavidin
3 Rhizavidin
4 Xenavidin
5 AVR2
6 AVR4

Avidin

3fdc, 2jgs, 2c4i, 1vyo, 1rav, 1ave – cAv – chicken
2a5b, 2a5c, 2a8g – cAv+deoxyribose
1nqn, 2cam – cAv (mutant)
1ldo, 1ldq, 1lel, 1ij8, 1avd, 2avi – cAV + biotin derivative

Strepavidin

1rxh – StrAv (mutant)+ biotinyl p-nitroaniline – Streptomyces avidinii
1rxj, 1rxk, 1nqm - StrAv (mutant)
1lcv, 1lcw, 1lcz, 1i9h – StrAv+biotin derivative

Rhizavidin

3ew1 – RhiAv – Rhizobium etli
3ew2 – RhiAV+biotin

Xenavidin

2uz2, 2uyw – XenAv – frog

AVR2

1wbi – cAVR2

AVR4

2fhl – cAVR4+biotinyl p-nitroaniline
2of9, 2ofa, 1y53 - cAVR4 (mutant)
2fhn, 2of8 - cAVR4 (mutant)+biotinyl p-nitroaniline
1y52, 1y55 - cAVR4 (mutant)+biotin