2jbm
From Proteopedia
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QPRTASE STRUCTURE FROM HUMAN
Overview
Human quinolinate phosphoribosyltransferase (EC 2.4.2.19) (hQPRTase) is a, member of the type II phosphoribosyltransferase family involved in the, catabolism of quinolinic acid (QA). It catalyses the formation of, nicotinic acid mononucleotide from quinolinic acid, which involves a, phosphoribosyl transfer reaction followed by decarboxylation. hQPRTase has, been implicated in a number of neurological conditions and in order to, study it further, we have carried out structural and kinetic studies on, recombinant hQPRTase. The structure of the fully active enzyme, overexpressed in Escherichia coli was solved using multiwavelength methods, to a resolution of 2.0 A. hQPRTase has a alpha/beta barrel fold sharing a, similar overall structure with the bacterial QPRTases. The active site of, hQPRTase is located at an alpha/beta open sandwich structure that serves, as a cup for the alpha/beta barrel of the adjacent subunit with a QA, binding site consisting of three arginine residues (R102, R138 and R161), and two lysine residues (K139 and K171). Mutation of these residues, affected substrate binding or abolished the enzymatic activity. The, kinetics of the human enzyme are different to the bacterial enzymes, studied, hQPRTase is inhibited competitively and non-competitively by one, of its substrates, 5-phosphoribosylpyrophosphate (PRPP). The human enzyme, adopts a hexameric arrangement, which places the active sites in close, proximity to each other.
About this Structure
2JBM is a Single protein structure of sequence from Homo sapiens with SRT as ligand. Active as Nicotinate-nucleotide diphosphorylase (carboxylating), with EC number 2.4.2.19 Known structural/functional Sites: , , , , , , , , , , and . Full crystallographic information is available from OCA.
Reference
Structural and Kinetic Characterization of Quinolinate Phosphoribosyltransferase (hQPRTase) from Homo sapiens., Liu H, Woznica K, Catton G, Crawford A, Botting N, Naismith JH, J Mol Biol. 2007 Oct 26;373(3):755-63. Epub 2007 Aug 24. PMID:17868694
Page seeded by OCA on Tue Dec 18 19:58:56 2007
