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2iu4

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Revision as of 15:54, 29 October 2007 by OCA (Talk | contribs)
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2iu4, resolution 1.96Å

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DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ

Overview

Dihydroxyacetone (Dha) kinases are a novel family of kinases with, signaling and metabolic functions. Here we report the x-ray structures of, the transcriptional activator DhaS and the coactivator DhaQ and, characterize their function. DhaQ is a paralog of the Dha binding Dha, kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional, activator. DhaQ and DhaS form a stable complex that in the presence of Dha, activates transcription of the Lactococcus lactis dha operon. Dha, covalently binds to DhaQ through a hemiaminal bond with a histidine and, thereby induces a conformational change, which is propagated to the, surface via a cantilever-like structure. DhaS binding protects an inverted, repeat whose sequence ... [(full description)]

About this Structure

2IU4 is a [Single protein] structure of sequence from [Lactococcus lactis] with SO4 and HIQ as [ligands]. Active as [[1]], with EC number [2.7.1.2]. Full crystallographic information is available from [OCA].

Reference

Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators., Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B, J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471

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