2ql6
From Proteopedia
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human nicotinamide riboside kinase (NRK1)
Overview
Nicotinamide riboside kinase (NRK) has an important role in the, biosynthesis of NAD(+) as well as the activation of tiazofurin and other, NR analogs for anticancer therapy. NRK belongs to the deoxynucleoside, kinase and nucleoside monophosphate (NMP) kinase superfamily, although the, degree of sequence conservation is very low. We report here the crystal, structures of human NRK1 in a binary complex with the reaction product, nicotinamide mononucleotide (NMN) at 1.5 A resolution and in a ternary, complex with ADP and tiazofurin at 2.7 A resolution. The active site is, located in a groove between the central parallel beta sheet core and the, LID and NMP-binding domains. The hydroxyl groups on the ribose of NR are, recognized by Asp56 and Arg129, and Asp36 is the general base of the, enzyme. Mutation of residues in the active site can abolish the catalytic, activity of the enzyme, confirming the structural observations.
About this Structure
2QL6 is a Single protein structure of sequence from Homo sapiens with ADP and TIZ as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of human nicotinamide riboside kinase., Khan JA, Xiang S, Tong L, Structure. 2007 Aug;15(8):1005-13. PMID:17698003
Page seeded by OCA on Mon Nov 12 23:33:31 2007
