2hz5

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 2hz5, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of human dynein light chain Dnlc2A

Overview

The human light chain of the motor protein dynein, Dnlc2A, is also a novel, TGF-beta-signaling component, which is altered with high frequency in, epithelial ovarian cancer. It is an important mediator of dynein and the, development of cancer, owing to its ability to bind to the dynein, intermediate light chain (DIC) IC74 and to regulate TGF-beta-dependent, transcriptional events. Here we report the 2.1-A crystal structure of, Dnlc2A using single anomalous diffraction. The proteins form a homodimer, in solution and interact mainly through the helix alpha(2), strand, beta(3), and the loop following this strand in each protein to generate a, 10-stranded beta-sheet core. The surface of the beta-sheet core is mainly, positively charged and predicted (by software PPI-Pred) to be the site, that interacts with other partners. At the same time, the residues 79-82, 88, and 90 of each molecule formed two holes in the core. Residue 89 of, each molecule, which is crucial for the DIC binding function of Dnlc2A, is, within the holes. On the basis of these observations, we propose that the, homodimer is the structural and functional unit maintained by hydrogen, bonding interactions and hydrophobic packing, and that the patch of the, surface of the beta-sheet core is the main area of interaction with other, partners. Furthermore, the two holes would be the key sites to interact, with IC74.

About this Structure

2HZ5 is a Single protein structure of sequence from Homo sapiens with CS as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of human dynein light chain Dnlc2A: structural insights into the interaction with IC74., Liu JF, Wang ZX, Wang XQ, Tang Q, An XM, Gui LL, Liang DC, Biochem Biophys Res Commun. 2006 Oct 27;349(3):1125-9. Epub 2006 Sep 5. PMID:16970917

Page seeded by OCA on Mon Nov 12 22:38:50 2007