2ccz
From Proteopedia
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CRYSTAL STRUCTURE OF E. COLI PRIMOSOMOL PROTEIN PRIB BOUND TO SSDNA
Overview
PriB is a primosomal protein required for replication restart in, Escherichia coli. PriB stimulates PriA helicase activity via interaction, with single-stranded DNA (ssDNA), but the molecular details of this, interaction remain unclear. Here, we report the crystal structure of PriB, complexed with a 15 bases oligonucleotide (dT15) at 2.7 A resolution. PriB, shares structural similarity with the E.coli ssDNA-binding protein, (EcoSSB). However, the structure of the PriB-dT15 complex reveals that, PriB binds ssDNA differently. Results from filter-binding assays show that, PriB-ssDNA interaction is salt-sensitive and cooperative. Mutational, analysis suggests that the loop L45 plays an important role in ssDNA, binding. Based on the crystal structure and biochemical analyses, we, propose a cooperative mechanism for the binding of PriB to ssDNA and a, model for the assembly of the PriA-PriB-ssDNA complex. This report, presents the first structure of a replication restart primosomal protein, complexed with DNA, and a novel model that explains the interactions, between a dimeric oligonucleotide-binding-fold protein and ssDNA.
About this Structure
2CCZ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode., Huang CY, Hsu CH, Sun YJ, Wu HN, Hsiao CD, Nucleic Acids Res. 2006;34(14):3878-86. Epub 2006 Aug 9. PMID:16899446
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