Sandbox88

From Proteopedia

Revision as of 13:09, 13 April 2011 by Scott Eron (Talk | contribs)
Jump to: navigation, search

From Proteopedia Jump to: navigation, search

This sandbox is in use until August 1, 2011 for UMass Chemistry 423. Others please do not edit this page. Thanks!



Caspase 7

This is the structure for Caspase 7 with substrate bound. Caspase 7 is a Cysteine dependent protease that cleaves after ASPartate residues. The substrate here is the peptide DEVD. Caspase 7 plays a critical role in apoptosis as an initiator that cleaves downstream targets.

All caspases are zymogens. In the case of Casp7, this means that there is a prodomain that, when attached, renders the protein inactive. However, this prodomain is easily cleaved off, thus activating Casp7 as a protease.


PDB ID 1f1j

Drag the structure with the mouse to rotate
1f1j, resolution 2.35Å ()
Ligands:
Non-Standard Residues: ,
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Active Site

The active site for Caspase 7 takes full advantage of acid base chemistry. Histidine 144 will act as a base, pulling the hydrogen away from cysteine 186. This will make that cysteine very nucleophilic and thus very active. It will bind substrate and cleave after an aspartate residue.

Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox88"
Personal tools