2oqu
From Proteopedia
|
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Overview
Crystal cryocooling is usually employed to reduce radiation damage during, X-ray crystallography. Recently, a high-pressure cryocooling method has, been developed which results in excellent diffraction-quality crystals, without the use of penetrative cryoprotectants. Three new developments of, the method are presented here: (i) Xe-He high-pressure cryocooling for Xe, SAD phasing, (ii) native sulfur SAD phasing and (iii) successful, cryopreservation of crystals in thick-walled capillaries without, additional cryoprotectants other than the native mother liquor. These, developments may be useful for structural solution of proteins without the, need for selenomethionine incorporation and for high-throughput protein, crystallography.
About this Structure
2OQU is a Single protein structure of sequence from Sus scrofa with CA, SO4 and XE as ligands. Active as Pancreatic elastase, with EC number 3.4.21.36 Full crystallographic information is available from OCA.
Reference
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791
Page seeded by OCA on Wed Nov 21 13:15:40 2007
Categories: Pancreatic elastase | Single protein | Sus scrofa | Gruner, S.M. | Hao, Q. | Kim, C.U. | CA | SO4 | XE | High pressure cryocooling | Sad phasing | Xenon
