Group:MUZIC:ALP

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Contents 1 ALP Subfamily: PDZ/LIM domain proteins of the cytoskeleton 2 Sequence annotation 3 Structure 4 Function and Interactions 5 Pathology 6 References

ALP Subfamily: PDZ/LIM domain proteins of the cytoskeleton

On the basis of structural similarities, the ALP (α-Actinin-associated LIM domain Proteins) subfamily are related to the Enigma subfamily [1], containing PDZ/LIM-domains, as well as internal motifs viz ALP-like motif (AM) and ZASP-like motif (ZM). However, the ALP subfamily differs from the Enigma subfamily as each member of the ALP subfamily possess an N-terminal PDZ domain and only one C-terminal LIM domain.

Four proteins: ALP (aka PDLIM3)^[1], CLP36^[2] (aka hCLIM1(human)^[3], Elfin (mouse)^[4], PDLIM1), RIL^[5] (aka PDLIM4) and Mystique^[6],^[7] (aka SLIM, PDLIM2) have been classified into the ALP subfamily.

CLP36 (C-terminal LIM Protein 36 kDa) and RIL (Reversion-Induced LIM) protein α-actinin-associated LIM protein (ALP) was first identified as a 39 kDa protein in rat skeletal muscle with a slightly shorter isoform in heart ^[8]. ALP includes both an N-terminal PDZ domain, which interacts with the a-actinin-2, and a C-terminal LIM domain. Thus, ALP shares a high degree of homology with previously characterized CLP-36 (Wang et al., 1995) and RIL (Kiess et al., 1995) proteins, suggesting a novel protein family containing an N-terminal PDZ domain and one C-terminal LIM motif (Xia et al., 1997).

Sequence annotation

Structure

Function and Interactions

Pathology

References

1. ↑ Xia H, Winokur ST, Kuo WL, Altherr MR, Bredt DS. Actinin-associated LIM protein: identification of a domain interaction between PDZ and spectrin-like repeat motifs. J Cell Biol. 1997 Oct 20;139(2):507-15. PMID:9334352
2. ↑ Wang H, Harrison-Shostak DC, Lemasters JJ, Herman B. Cloning of a rat cDNA encoding a novel LIM domain protein with high homology to rat RIL. Gene. 1995 Nov 20;165(2):267-71. PMID:8522188
3. ↑ Kotaka M, Ngai SM, Garcia-Barcelo M, Tsui SK, Fung KP, Lee CY, Waye MM. Characterization of the human 36-kDa carboxyl terminal LIM domain protein (hCLIM1). J Cell Biochem. 1999 Feb 1;72(2):279-85. PMID:10022510
4. ↑ Kotaka M, Lau YM, Cheung KK, Lee SM, Li HY, Chan WY, Fung KP, Lee CY, Waye MM, Tsui SK. Elfin is expressed during early heart development. J Cell Biochem. 2001 Aug 21-Sep 5;83(3):463-72. PMID:11596114
5. ↑ Kiess M, Scharm B, Aguzzi A, Hajnal A, Klemenz R, Schwarte-Waldhoff I, Schafer R. Expression of ril, a novel LIM domain gene, is down-regulated in Hras-transformed cells and restored in phenotypic revertants. Oncogene. 1995 Jan 5;10(1):61-8. PMID:7824279
6. ↑ Torrado M, Senatorov VV, Trivedi R, Fariss RN, Tomarev SI. Pdlim2, a novel PDZ-LIM domain protein, interacts with alpha-actinins and filamin A. Invest Ophthalmol Vis Sci. 2004 Nov;45(11):3955-63. PMID:15505042 doi:10.1167/iovs.04-0721
7. ↑ Loughran G, Healy NC, Kiely PA, Huigsloot M, Kedersha NL, O'Connor R. Mystique is a new insulin-like growth factor-I-regulated PDZ-LIM domain protein that promotes cell attachment and migration and suppresses Anchorage-independent growth. Mol Biol Cell. 2005 Apr;16(4):1811-22. Epub 2005 Jan 19. PMID:15659642 doi:10.1091/mbc.E04-12-1052
8. ↑ Xia H, Winokur ST, Kuo WL, Altherr MR, Bredt DS. Actinin-associated LIM protein: identification of a domain interaction between PDZ and spectrin-like repeat motifs. J Cell Biol. 1997 Oct 20;139(2):507-15. PMID:9334352