1gxd
From Proteopedia
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PROMMP-2/TIMP-2 COMPLEX
Contents |
Overview
Matrix metalloproteinases (MMPs) are a family of multidomain enzymes, involved in the physiological degradation of connective tissue, as well as, in pathological states such as tumor invasion and arthritis. Apart from, transcriptional regulation, MMPs are controlled by proenzyme activation, and a class of specific tissue inhibitors of metalloproteinases (TIMPs), that bind to the catalytic site. TIMP-2 is a potent inhibitor of MMPs, but, it has also been implicated in a unique cell surface activation mechanism, of latent MMP-2/gelatinase A/type IV collagenase (proMMP-2), through its, binding to the hemopexin domain of proMMP-2 on the one hand and to a, membrane-type MMP activator on the other. The present crystal structure of, the human proMMP-2/TIMP-2 complex reveals an interaction between the, hemopexin domain of proMMP-2 and the C-terminal domain of TIMP-2, leaving, the catalytic site of MMP-2 and the inhibitory site of TIMP-2 distant and, spatially isolated. The interfacial contact of these two proteins is, characterized by two distinct binding regions composed of alternating, hydrophobic and hydrophilic interactions. This unique structure provides, information for how specificity for noninhibitory MMP/TIMP complex, formation is achieved.
Disease
Known diseases associated with this structure: Osteolysis, idiopathic, Saudi type OMIM:[120360], Winchester syndrome OMIM:[120360]
About this Structure
1GXD is a Protein complex structure of sequences from Homo sapiens with SO4, ZN and CA as ligands. Active as Gelatinase A, with EC number 3.4.24.24 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural insight into the complex formation of latent matrix metalloproteinase 2 with tissue inhibitor of metalloproteinase 2., Morgunova E, Tuuttila A, Bergmann U, Tryggvason K, Proc Natl Acad Sci U S A. 2002 May 28;99(11):7414-9. PMID:12032297
Page seeded by OCA on Tue Dec 18 15:44:12 2007
