User:Mitchell Long/Sandbox 1
From Proteopedia
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| 3fgc, resolution 2.30Å () | |||||||||
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| Ligands: | , , | ||||||||
| Gene: | luxA (Vibrio harveyi), luxB (Vibrio harveyi) | ||||||||
| Activity: | Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3 | ||||||||
| Related: | 1luc, 1brl | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Mechanism of Bio luminescence
Luciferase found in 'Italic tex
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| 3fgc, resolution 2.30Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||||
| Gene: | luxA (Vibrio harveyi), luxB (Vibrio harveyi) | ||||||||
| Activity: | Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3 | ||||||||
| Related: | 1luc, 1brl | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Mechanism of Bio luminescence
Luciferase found in 'Vibrio harveyi' is a heterodimeric flavin monoxygenase that is composed of a catalytic α subunit and a homologous but noncatalytic β subunit. The catalytic α subunit is bound to a Flavin Mononucleotide cofactor. Bioluminescense is achieved by a two step reaction between
Structural Motifs
Active Site
Mobile Loop-The mobile loop of luciferase is thought to be responsible for protecting reaction intermediates and preventing the entry of bulk solvent into the active center once the flavin substrate is bound.
(β/α)&sub8; Barrel-
Interface between Alpha and Beta Subunits
Applications In Biotechnology
The luciferase assay
Quorum Sensing
Quorum sensing is a population density dependent form gene expression found in many bacteria.
