This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2c98
From Proteopedia
|
STRUCTURAL BASIS OF THE NUCLEOTIDE DRIVEN CONFORMATIONAL CHANGES IN THE AAA DOMAIN OF TRANSCRIPTION ACTIVATOR PSPF
Overview
Bacterial enhancer-binding proteins (EBP) activate transcription by, hydrolyzing ATP to restructure the sigma(54)-RNA polymerase-promoter, complex. We compare six high resolution structures (<2.1 A) of the AAA(+), domain of EBP phage shock protein F (PspF) including apo, AMPPNP, Mg(2+)-ATP, and ADP forms. These structures permit a description of the, atomic details underpinning the origins of the conformational changes, occurring during ATP hydrolysis. Conserved regions of PspF's AAA(+) domain, respond distinctively to nucleotide binding and hydrolysis, suggesting, functional roles during the hydrolysis cycle, which completely agree with, those derived from activities of PspF mutated at these positions. We, propose a putative atomic switch that is responsible for coupling, structural changes in the nucleotide-binding site to the repositioning of, the sigma(54)-interacting loops. Striking similarities in, nucleotide-specific conformational changes and atomic switch exist between, PspF and the large T antigen helicase, suggesting conservation in the, origin of those events amongst AAA(+) proteins.
About this Structure
2C98 is a Single protein structure of sequence from Escherichia coli with ADP as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural basis of the nucleotide driven conformational changes in the AAA+ domain of transcription activator PspF., Rappas M, Schumacher J, Niwa H, Buck M, Zhang X, J Mol Biol. 2006 Mar 24;357(2):481-92. Epub 2006 Jan 13. PMID:16430918
Page seeded by OCA on Tue Dec 18 19:14:41 2007
Categories: Escherichia coli | Single protein | Buck, M. | Niwa, H. | Rappas, M. | Schumacher, J. | Zhang, X. | ADP | Aaa domain | Activator | Atp-binding | Atpase | Bacterial sigma54 activator | Dna-binding | Nucleotide-binding | Sensory transduction | Transcription regulation | Two-component regulatory system
