Sandbox 210

From Proteopedia

proteopedia linkproteopedia link

Contents 1 CDK5-p25 complex 2 Discovery of CDK5 3 Structure 3.1 Sequence similarities 4 Applications of CDK5-p25 complex 5 Biology 5.1 CDK5-p25 complex Control 6 References

CDK5-p25 complex

spinqualitylabelsall models PDB ID 1h4l Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
1h4l, resolution 2.65Å ()
Related:	1lfr
Structural annotation: Resources: CATH : 1H4La02, 1H4La01, 1H4Lb01, 1H4Lb02, 1H4Ld00, 1H4Le00 InterPro : Ipr008271, Ipr011009, Ipr000719, Ipr002290, Ipr013763, Ipr004944, Ipr011028 Pfam : PF00069, PF03261 SCOP : d1h4la_, d1h4lb_, d1h4ld_, d1h4le_ UniProt : Q00535, Q15078
Functional annotation: Cellular component: nucleus dendrite growth cone cell soma neuromuscular junction lamellipodium cell projection membrane cytosol axon cytoplasm filopodium dendritic spine cyclin-dependent protein kinase 5 activator complex contractile fiber Biological process: neuron differentiation cell migration embryonic development axonogenesis neurite development cell division cell proliferation protein amino acid phosphorylation regulation of cell migration positive regulation of neuron apoptosis cell cycle cell-matrix adhesion neuron migration ionotropic glutamate receptor signaling pathway brain development neuron adhesion regulation of cyclin-dependent protein kinase activity axon guidance regulation of neuron differentiation axonal fasciculation acetylcholine receptor signaling, muscarinic pathway Molecular function: ErbB-3 class receptor binding cyclin-dependent protein kinase activity ATP binding nucleotide binding transferase activity protein binding ErbB-2 class receptor binding protein kinase activity acetylcholine receptor activator activity kinase activity tau-protein kinase activity protein serine/threonine kinase activity cadherin binding calcium ion binding cyclin-dependent protein kinase 5 activator activity protein serine/threonine kinase activator activity
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

CDK means Cyclin Dependent Kinase. The cyclin-dependent kinases (CDKs) are a large family of serine–threonine kinases which control the eukaryotic cell cycle, when they are activated by the cyclin regulatory subunit. These kinases phosphorylate a very large number of protein in order to inactivate or activate them.^[1]

Cyclin dependent kinase 5(CDK5) is a unique member of CDK family members that is not activated by a cyclin. Instead it is activated by a kinase, p35 also named Cyclin-dependent kinase 5 activator. Unlike other CDKs, it plays a major role in cell communication, cell morphology and motility.

CDK5 could form a complex with p25, an other protein.

Discovery of CDK5

Reported by four independent groups of scientists in 1992, Cdk5 had multiple names. A first group reported it as a neuronal Cdc2 like kinase (NCLK) characterized and cloned from rat brain c-DNA library as a Cdk that phosphorylates the lysine–serine–proline motif of neurofilaments. It was reported to have 58% amino acid sequence homology with mouse Cdk1 and 61% homology with human Cdk2. While looking for several Cdks, which play an important role in eukaryotic cell cycle, a second group found Cdk5/PSSALARE kinase (with an alpha C helix) among several other Cdks. A third group of scientists called the same molecule as brain proline-directed protein kinase (BPDK), reporting it in bovine brain with functional similarity to cdc2. In the same year, the fourth group named it as Tau protein kinase II (TPK II) associated with microtubules. Later in 1993, the nomenclature settled down to Cdk5 after identifying the 30 kDa protein subunit of the active enzyme.^[2]

Structure

The subunit of the CDK5 shares also a very similar three-dimensional (3D) structure with CDK2.

Sequence similarities

The of the CDK5-p25 complex share homologuous sequences with the cell division protein kinase 5 and the with the cyclin-dependent kinase 5 activator (p35).

Applications of CDK5-p25 complex

Biology

CDK5-p25 complex Control

References

1. ↑ Zhang B, Su ZC, Tay TE, Tan VB. Mechanism of CDK5 activation revealed by steered molecular dynamics simulations and energy calculations. J Mol Model. 2010 Jun;16(6):1159-68. Epub 2009 Dec 15. PMID:20013135 doi:10.1007/s00894-009-0629-4
2. ↑ Dhariwala FA, Rajadhyaksha MS. An unusual member of the Cdk family: Cdk5. Cell Mol Neurobiol. 2008 May;28(3):351-69. Epub 2008 Jan 8. PMID:18183483 doi:10.1007/s10571-007-9242-1