2j5m
From Proteopedia
|
STRUCTURE OF CHLOROPEROXIDASE COMPOUND 0
Overview
We have determined the crystal structure of the chloroperoxidase (CPO), hydroperoxo reaction intermediate (CPO compound 0) at 1.75-A resolution., The intermediate was generated through controlled photoreduction of the, CPO oxygen complex during x-ray data collection, which was monitored by, recording of the crystal absorption spectra. Initially, the peroxo-anion, species was formed and then protonated to yield compound 0. Quantum, chemical calculations indicate that the peroxo-anion species is not stable, and collapses instantaneously to compound 0. Compound 0 is present in the, ferric low-spin doublet ground state and is characterized by a long O O, bond length of 1.5 A and a Fe O bond distance of 1.8 A, which is also, observed in the crystal structure.
About this Structure
2J5M is a Single protein structure of sequence from Leptoxyphium fumago with NAG, MAN, MN, ACT, HEM, PEO and EDO as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and quantum chemical characterization of chloroperoxidase compound 0, a common reaction intermediate of diverse heme enzymes., Kuhnel K, Derat E, Terner J, Shaik S, Schlichting I, Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):99-104. Epub 2006 Dec 26. PMID:17190816
Page seeded by OCA on Tue Dec 18 19:49:46 2007
Categories: Chloride peroxidase | Leptoxyphium fumago | Single protein | Derat, E. | Kuhnel, K. | Schlichting, I. | Shaik, S. | Terner, J. | ACT | EDO | HEM | MAN | MN | NAG | PEO | Chloride | Glycoprotein | Heme | Iron | Manganese | Metal-binding | Oxidoreductase | Peroxidase | Pyrrolidone carboxylic acid
