1lpk
From Proteopedia
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CRYSTAL STRUCTURE OF FXA IN COMPLEX WITH 125.
Contents |
Overview
A series of 138 nonchiral 3-amidinobenzyl-1H-indole-2-carboxamides and, analogues as inhibitors of the blood coagulation enzyme factor Xa (fXa), were designed, synthesized, and investigated by X-ray structure analysis, and 3D quantitative structure-activity relationship (QSAR) studies (CoMFA, CoMSIA) in order to identify important protein-ligand interactions, responsible for biological affinity and selectivity. Several compounds, from this series are highly potent and selective inhibitors of this, important enzyme linking extrinsic and intrinsic coagulation pathways. To, rationalize biological affinity and to provide guidelines for further, design, all compounds were docked into the factor Xa binding site. Those, docking studies were based on X-ray structures of factor Xa in complex, with literature-known inhibitors. It was possible to validate those, binding modes by four X-ray crystal structures of representative ligands, in factor Xa, while one ligand was additionally crystallized in trypsin to, rationalize requirements for selective factor Xa inhibition. The 3D-QSAR, models based on a superposition rule derived from these docking studies, were validated using conventional and cross-validated r(2) values using, the leave-one-out method and repeated analyses using two randomly chosen, cross-validation groups plus randomization of biological activities. This, led to consistent and highly predictive 3D-QSAR models with good, correlation coefficients for both CoMFA and CoMSIA, which were found to, correspond to experimentally determined factor Xa binding site topology in, terms of steric, electrostatic, and hydrophobic complementarity. Subsets, selected as smaller training sets using 2D fingerprints and maximum, dissimilarity methods resulted in 3D-QSAR models with remarkable, correlation coefficients and a high predictive power. The final, quantitative SAR information agrees with all experimental data for the, binding topology and thus provides reasonable activity predictions for, novel factor Xa inhibitors.
Disease
Known disease associated with this structure: Factor X deficiency OMIM:[227600]
About this Structure
1LPK is a Protein complex structure of sequences from Homo sapiens with CA and CBB as ligands. Active as Coagulation factor Xa, with EC number 3.4.21.6 Full crystallographic information is available from OCA.
Reference
Design and quantitative structure-activity relationship of 3-amidinobenzyl-1H-indole-2-carboxamides as potent, nonchiral, and selective inhibitors of blood coagulation factor Xa., Matter H, Defossa E, Heinelt U, Blohm PM, Schneider D, Muller A, Herok S, Schreuder H, Liesum A, Brachvogel V, Lonze P, Walser A, Al-Obeidi F, Wildgoose P, J Med Chem. 2002 Jun 20;45(13):2749-69. PMID:12061878
Page seeded by OCA on Mon Nov 12 18:03:18 2007
