1qg7
From Proteopedia
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STROMA CELL-DERIVED FACTOR-1ALPHA (SDF-1ALPHA)
Contents |
Overview
Crystal structures, forms 1 and 2, of recombinant native stromal, cell-derived factor-1alpha (SDF-1alpha), expressed using the Sendai virus, expression vector system, have been determined by x-ray crystallography at, 2.0 A resolution. The crystal of form 1 is almost isomorphous with that, used in the previous crystal structure analysis of the synthetic [N33A], mutant of SDF-1alpha (Dealwis, C., et al. Proc. Natl. Acad. Sci. USA, 1998;95, 6941-6946). However, the present structure analysis led to, considerably better refinement statistics, revealing an error in the, structural assignment of N-terminal residues in the previous report., Comparison of the solution structure, as previously determined by nuclear, magnetic resonance (NMR) spectroscopy, and the present structure, with two, monomers in the asymmetric unit, reveals several local conformational, differences. Alanine scan mutagenesis studies for each residue in the, so-called RFFESH motif revealed that only the first residue, Arg12, is, effective in enhancing receptor binding (and successive activation). A new, notion that steric restraint between Arg8 and Arg12 is favorable (if not, vital) for retaining SDF activities appears to explain more consistently, the structure-activity relationship data accumulated to date. Four guiding, principles are presented that may be useful for designing potent, therapeutic compounds interfering with HIV-1 infection through competition, at the CXCR4 coreceptor.
Disease
Known diseases associated with this structure: AIDS, resistance to OMIM:[600835]
About this Structure
1QG7 is a Single protein structure of sequence from Homo sapiens with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of recombinant native SDF-1alpha with additional mutagenesis studies: an attempt at a more comprehensive interpretation of accumulated structure-activity relationship data., Ohnishi Y, Senda T, Nandhagopal N, Sugimoto K, Shioda T, Nagal Y, Mitsui Y, J Interferon Cytokine Res. 2000 Aug;20(8):691-700. PMID:10954912
Page seeded by OCA on Mon Nov 12 18:52:53 2007
