1a3m
From Proteopedia
|
PAROMOMYCIN BINDING INDUCES A LOCAL CONFORMATIONAL CHANGE IN THE A SITE OF 16S RRNA, NMR, 20 STRUCTURES
Overview
Aminoglycoside antibiotics that bind to ribosomal RNA in the, aminoacyl-tRNA site (A-site) cause misreading of the genetic code and, inhibit translocation. An A-site RNA oligonucleotide specifically binds to, aminoglycoside antibiotics and the structure of the RNA-paromomycin, complex was previously determined by nuclear magnetic resonance (NMR), spectroscopy. Here, the A-site RNA structure in its free form has been, determined using heteronuclear NMR and compared to the structure of the, paromomycin-RNA complex. As in the complex with paromomycin, the, asymmetric internal loop is closed by a Watson-Crick base-pair, (C1407.G1494) and by two non-canonical base-pairs (U1406.U1495, A1408.A1493). A1492 stacks below A1493 and is intercalated between the, upper and lower stems. The comparison of the free and bound conformations, of the RNA shows that two universally conserved residues of the A site of, 16 S rRNA, A1492 and A1493, are displaced towards the minor groove of the, RNA helix in presence of antibiotic. These changes in the RNA conformation, place the N1 positions of A1492 and A1493 on the minor groove side of the, A-site RNA and suggest a mechanism of action of aminoglycosides on, translation.
About this Structure
1A3M is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA., Fourmy D, Yoshizawa S, Puglisi JD, J Mol Biol. 1998 Mar 27;277(2):333-45. PMID:9514734
Page seeded by OCA on Sat Nov 24 22:21:45 2007
