1ag6

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spinqualitylabelsall models 1ag6, resolution 1.6Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PLASTOCYANIN FROM SPINACH

Overview

The crystal structure of plastocyanin from spinach has been determined, using molecular replacement, with the structure of plastocyanin from, poplar as a search model. Successful crystallization was facilitated by, site-directed mutagenesis in which residue Gly8 was substituted with Asp., The region around residue 8 was believed to be too mobile for the, wild-type protein to form crystals despite extensive screening. The, current structure represents the oxidized plastocyanin, copper (II), at, low pH (approximately 4.4). In contrast to the similarity in the core, region as compared to its poplar counterpart, the structure shows some, significant differences in loop regions. The most notable is the large, shift of the 59-61 loop where the largest shift is 3.0 A for the C(alpha), atom of Glu59. This results in different patterns of electrostatic, potential around the acidic patches for the two proteins.

About this Structure

1AG6 is a Single protein structure of sequence from Spinacia oleracea with CU as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:9792096

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