1bcu
From Proteopedia
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ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN
Contents |
Overview
Proflavin can be used to study the interactions of inhibitors and, substrates with thrombin by monitoring the changes in the visible, absorption spectrum that occur on dye displacement. We have used, microspectrophotometric methods to investigate the binding of proflavin to, crystals of an alpha-thrombin-hirugen complex and have determined the, structure by X-ray crystallography. The proflavin molecule binds in the S1, pocket of the enzyme with one of the amino groups hydrogen bonded to the, carboxylate of Asp-189 while the protonated ring nitrogen is hydrogen, bonded to the carbonyl of Gly-219. This result indicates that the, proflavin displacement assay can be used to specifically monitor the, binding of inhibitors to the S1 pocket.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
1BCU is a Protein complex structure of sequences from Hirudo medicinalis and Homo sapiens with as ligand. Active as Thrombin, with EC number 3.4.21.5 Full crystallographic information is available from OCA.
Reference
X-ray and spectrophotometric studies of the binding of proflavin to the S1 specificity pocket of human alpha-thrombin., Conti E, Rivetti C, Wonacott A, Brick P, FEBS Lett. 1998 Mar 27;425(2):229-33. PMID:9559654
Page seeded by OCA on Fri Feb 15 15:31:49 2008
