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1bg5
From Proteopedia
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CRYSTAL STRUCTURE OF THE ANKYRIN BINDING DOMAIN OF ALPHA-NA,K-ATPASE AS A FUSION PROTEIN WITH GLUTATHIONE S-TRANSFERASE
Overview
The ankyrin 33-residue repeating motif, an L-shaped structure with, protruding beta-hairpin tips, mediates specific macromolecular, interactions with cytoskeletal, membrane, and regulatory proteins. The, association between ankyrin and alpha-Na,K-ATPase, a ubiquitous membrane, protein critical to vectorial transport of ions and nutrients, is required, to assemble and stabilize Na,K-ATPase at the plasma membrane., alpha-Na,K-ATPase binds both red cell ankyrin (AnkR, a product of the ANK1, gene) and Madin-Darby canine kidney cell ankyrin (AnkG, a product of the, ANK3 gene) utilizing residues 142-166 (SYYQEAKSSKIMESFK NMVPQQALV) in its, second cytoplasmic domain. Fusion peptides of glutathione S-transferase, incorporating these 25 amino acids bind specifically to purified ankyrin, (Kd = 118 +/- 50 nM). The three-dimensional structure (2.6 A) of this, minimal ankyrin-binding motif, crystallized as the fusion protein, reveals, a 7-residue loop with one charged hydrophilic face capping a double, beta-strand. Comparison with ankyrin-binding sequences in p53, CD44, neurofascin/L1, and the inositol 1,4,5-trisphosphate receptor suggests, that the valency and specificity of ankyrin binding is achieved by the, interaction of 5-7-residue surface loops with the beta-hairpin tips of, multiple ankyrin repeat units.
About this Structure
1BG5 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure of the ankyrin-binding domain of alpha-Na,K-ATPase., Zhang Z, Devarajan P, Dorfman AL, Morrow JS, J Biol Chem. 1998 Jul 24;273(30):18681-4. PMID:9668035
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